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Cold antibody capture in a hematology laboratory while performing a blood count.
STÁRKOVÁ, Michaela
In my Bachelor thesis I followed up cold antibodies capture in a hematology laboratory during the blood cell count statement. The main goal of this thesis was to point out the possible influence of blood counts through the cold antibodies, practical laboratory methods - measurement of blood count values with hematological Abbott analyser, furthermore influencing the results of setting the cold antibodies through preanalytical phase and finally the assessment of received results in amount of 20 patients. The first part of this thesis focuses on general information of imunohematology which describes antigen, antibodies, structure of immunoglobulins. Moreover I dealt with physiology and blood composition, the blood counts and its parameters. In the end of this thesis there are information of autoimmune hemolytic anemia and cold antibodies including their preanalytical, analytical and postanalytical phase. The methodological part of this thesis was carried out in Biochemical-hematological laboratory Stafila s.r.o. All my measurements were made under the supervisory of hematological laboratory assistant. Stating the blood count was made with 24 patients through multiparametric fully automatic Abbott analyser. In that part, I firstly describe analyser specifications. In the following step I indicate the working aids which were used. Finally, there is described the process of the measurements in detail. Within the assessment of the measured values the main attention was paid to red blood line, especially influencing the values of erythrocyte, the hemoglobin, MCH and MCHC. In another part of this thesis are all the blood counts calibres of 24 samples, which I entered into graphs and charts. Out of all 24 measured values I can evaluate that only 4 samples were of unusual values of red line, which can indicate the presence of cold antibodies.
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